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zetaview particle metrix pmx-120  (Particle Metrix)

 
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    Structured Review

    Particle Metrix zetaview particle metrix pmx-120
    Zetaview Particle Metrix Pmx 120, supplied by Particle Metrix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/zetaview+particle+metrix+pmx-120/zetaview+pmx+110/pm40473132-77-4-5
    Average 90 stars, based on 1 article reviews
    zetaview particle metrix pmx-120 - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Negative Staining:

    Article Title: Association of TRPV1 and the SIRT3/SOD2 Signaling Pathway in Mononuclear Cells and Astrocyte-Derived Extracellular Vesicles in Patients with Schizophrenia.
    Article Snippet: .. This was followed by negative staining with a uranyl acetate solution for 1 min. Filter paper was used to remove the excess liquid, and the exosome morphology was examined and photographed under a TEM. (2) Nanoparticle tracking analysis: The size (nm) and concentration (particles/mL) of the ADEs were quantified using the ZetaView Particle Metrix (PMX-120, Particle Metrix, Meerbusch, Germany) instrument, and NTA 3.4.003 nanoparticle tracking software was used for the analysis. (3) Western blotting was performed to detect the three exosome markers: TSG101 (1:1000, Beyotime, cat. no. AF8259), CD81 (1:1000, Beyotime, cat. no. AG1530), and ALIX (1:1000, Beyotime, cat. no. AG4522). ..

    Transmission Electron Microscopy:

    Article Title: Association of TRPV1 and the SIRT3/SOD2 Signaling Pathway in Mononuclear Cells and Astrocyte-Derived Extracellular Vesicles in Patients with Schizophrenia.
    Article Snippet: .. This was followed by negative staining with a uranyl acetate solution for 1 min. Filter paper was used to remove the excess liquid, and the exosome morphology was examined and photographed under a TEM. (2) Nanoparticle tracking analysis: The size (nm) and concentration (particles/mL) of the ADEs were quantified using the ZetaView Particle Metrix (PMX-120, Particle Metrix, Meerbusch, Germany) instrument, and NTA 3.4.003 nanoparticle tracking software was used for the analysis. (3) Western blotting was performed to detect the three exosome markers: TSG101 (1:1000, Beyotime, cat. no. AF8259), CD81 (1:1000, Beyotime, cat. no. AG1530), and ALIX (1:1000, Beyotime, cat. no. AG4522). ..

    Concentration Assay:

    Article Title: Association of TRPV1 and the SIRT3/SOD2 Signaling Pathway in Mononuclear Cells and Astrocyte-Derived Extracellular Vesicles in Patients with Schizophrenia.
    Article Snippet: .. This was followed by negative staining with a uranyl acetate solution for 1 min. Filter paper was used to remove the excess liquid, and the exosome morphology was examined and photographed under a TEM. (2) Nanoparticle tracking analysis: The size (nm) and concentration (particles/mL) of the ADEs were quantified using the ZetaView Particle Metrix (PMX-120, Particle Metrix, Meerbusch, Germany) instrument, and NTA 3.4.003 nanoparticle tracking software was used for the analysis. (3) Western blotting was performed to detect the three exosome markers: TSG101 (1:1000, Beyotime, cat. no. AF8259), CD81 (1:1000, Beyotime, cat. no. AG1530), and ALIX (1:1000, Beyotime, cat. no. AG4522). ..

    Article Title: Mesenchymal Stem Cell-Loaded Hydrogel Improves Surgical Treatment for Chronic Cerebral Ischemia.
    Article Snippet: Chronic cerebral ischemia (CCI) results in a prolonged insufficient blood supply to the brain tissue, leading to impaired neuronal function and subsequent impairment of cognitive and motor abilities.. Our previous research showed that in mice with bilateral carotid artery stenosis, the collateral neovascularization post Encephalo-myo-synangiosis (EMS) treatment could be facilitated by bone marrow mesenchymal stem cells (MSCs) transplantation.. Considering the advantages of biomaterials, we synthesized and modified a gelatin hydrogel for MSCs encapsulation.

    Software:

    Article Title: Association of TRPV1 and the SIRT3/SOD2 Signaling Pathway in Mononuclear Cells and Astrocyte-Derived Extracellular Vesicles in Patients with Schizophrenia.
    Article Snippet: .. This was followed by negative staining with a uranyl acetate solution for 1 min. Filter paper was used to remove the excess liquid, and the exosome morphology was examined and photographed under a TEM. (2) Nanoparticle tracking analysis: The size (nm) and concentration (particles/mL) of the ADEs were quantified using the ZetaView Particle Metrix (PMX-120, Particle Metrix, Meerbusch, Germany) instrument, and NTA 3.4.003 nanoparticle tracking software was used for the analysis. (3) Western blotting was performed to detect the three exosome markers: TSG101 (1:1000, Beyotime, cat. no. AF8259), CD81 (1:1000, Beyotime, cat. no. AG1530), and ALIX (1:1000, Beyotime, cat. no. AG4522). ..

    Western Blot:

    Article Title: Association of TRPV1 and the SIRT3/SOD2 Signaling Pathway in Mononuclear Cells and Astrocyte-Derived Extracellular Vesicles in Patients with Schizophrenia.
    Article Snippet: .. This was followed by negative staining with a uranyl acetate solution for 1 min. Filter paper was used to remove the excess liquid, and the exosome morphology was examined and photographed under a TEM. (2) Nanoparticle tracking analysis: The size (nm) and concentration (particles/mL) of the ADEs were quantified using the ZetaView Particle Metrix (PMX-120, Particle Metrix, Meerbusch, Germany) instrument, and NTA 3.4.003 nanoparticle tracking software was used for the analysis. (3) Western blotting was performed to detect the three exosome markers: TSG101 (1:1000, Beyotime, cat. no. AF8259), CD81 (1:1000, Beyotime, cat. no. AG1530), and ALIX (1:1000, Beyotime, cat. no. AG4522). ..

    Analysis:

    Article Title: Mesenchymal Stem Cell-Loaded Hydrogel Improves Surgical Treatment for Chronic Cerebral Ischemia.
    Article Snippet: Chronic cerebral ischemia (CCI) results in a prolonged insufficient blood supply to the brain tissue, leading to impaired neuronal function and subsequent impairment of cognitive and motor abilities.. Our previous research showed that in mice with bilateral carotid artery stenosis, the collateral neovascularization post Encephalo-myo-synangiosis (EMS) treatment could be facilitated by bone marrow mesenchymal stem cells (MSCs) transplantation.. Considering the advantages of biomaterials, we synthesized and modified a gelatin hydrogel for MSCs encapsulation.

    other:

    Article Title: Characterization of microRNA and Metabolite Profiles of Seminal Extracellular Vesicles in Boars.
    Article Snippet: The size of EVs was measured by nanoparticle tracking analysis (NTA) using a ZetaView Particle Metrix (PMX-120, Meerbusch, Germany).



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    40,000 × g is sufficient to pellet the majority of plant EVs. Immunoblots, nanoparticle tracking analysis (NTA), and representative transmission electron microscopy (TEM) images of the indicated extracellular fractions from Arabidopsis rosettes processed by differential ultracentrifugation, as indicated in the diagram. For immunoblots, samples are apoplastic wash fluid (AWF) and the indicated pellets collected from 5 mL of AWF. Numbers on the left sides of immunoblots indicate sizes in kiloDaltons. The TET8 antibody strongly cross‐reacts with a <25 kDa protein abundant in EV pellets. The arrowhead indicates the TET8‐specific band that corresponds to the predicted molecular weight of the protein and is verified by probing a tet8 null mutant (Figure ). The prominent band in Ponceau S at 55 kDa is likely RBCL. For TEM, EV, extracellular vesicle; nf, nanofilament; sip, small irregular particle <50 nm in diameter. Arabidopsis expressing RFP‐PEN1, TET8‐GFP and GFP‐REMORIN1.3 (REM1.3) were used where indicated. P10 = pellet from 10,000 × g centrifugation of AWF; P40 = pellet from 40,000 × g centrifugation of P10 supernatant; P100‐40 = pellet from 100,000 × g centrifugation of P40 supernatant; P100 = pellet from 100,000 × g centrifugation from P10 supernatant (no 40,000 × g step); P400 = pellet from 400,000 × g centrifugation of P100 supernatant; S100 = supernatant after 100,000 × g centrifugation.

    Journal: Journal of Extracellular Vesicles

    Article Title: Arabidopsis Produces Distinct Subpopulations of Extracellular Vesicles That Respond Differentially to Biotic Stress, Altering Growth and Infectivity of a Fungal Pathogen

    doi: 10.1002/jev2.70090

    Figure Lengend Snippet: 40,000 × g is sufficient to pellet the majority of plant EVs. Immunoblots, nanoparticle tracking analysis (NTA), and representative transmission electron microscopy (TEM) images of the indicated extracellular fractions from Arabidopsis rosettes processed by differential ultracentrifugation, as indicated in the diagram. For immunoblots, samples are apoplastic wash fluid (AWF) and the indicated pellets collected from 5 mL of AWF. Numbers on the left sides of immunoblots indicate sizes in kiloDaltons. The TET8 antibody strongly cross‐reacts with a <25 kDa protein abundant in EV pellets. The arrowhead indicates the TET8‐specific band that corresponds to the predicted molecular weight of the protein and is verified by probing a tet8 null mutant (Figure ). The prominent band in Ponceau S at 55 kDa is likely RBCL. For TEM, EV, extracellular vesicle; nf, nanofilament; sip, small irregular particle <50 nm in diameter. Arabidopsis expressing RFP‐PEN1, TET8‐GFP and GFP‐REMORIN1.3 (REM1.3) were used where indicated. P10 = pellet from 10,000 × g centrifugation of AWF; P40 = pellet from 40,000 × g centrifugation of P10 supernatant; P100‐40 = pellet from 100,000 × g centrifugation of P40 supernatant; P100 = pellet from 100,000 × g centrifugation from P10 supernatant (no 40,000 × g step); P400 = pellet from 400,000 × g centrifugation of P100 supernatant; S100 = supernatant after 100,000 × g centrifugation.

    Article Snippet: The nanoparticle tracker (Particle Metrix PMX 120 Zetaview Mono 488 Laser running Zetaview software version 8.05.16 SP3) was calibrated with 100 nm beads (nanoComposix silica nanospheres #SISN100).

    Techniques: Western Blot, Transmission Assay, Electron Microscopy, Molecular Weight, Mutagenesis, Expressing, Centrifugation

    Identification of the exosomes (Exos). A. Shape of the particles observed under a transmission electron microscope; B. Particle size distribution of the exosomes examined by nanoparticle tracking analysis; C. Expression of the exosome-specific biomarkers CD9 and CD81 examined by western blot analysis. Three independent experiments were performed.

    Journal: American Journal of Translational Research

    Article Title: Protective effect of endothelial progenitor cell-derived exosomal microRNA-382-3p on sepsis-induced organ damage and immune suppression in mice

    doi:

    Figure Lengend Snippet: Identification of the exosomes (Exos). A. Shape of the particles observed under a transmission electron microscope; B. Particle size distribution of the exosomes examined by nanoparticle tracking analysis; C. Expression of the exosome-specific biomarkers CD9 and CD81 examined by western blot analysis. Three independent experiments were performed.

    Article Snippet: The exosomes were resuspended in PBS and processed using nanoparticle tracking analysis (NTA) particle Metrix equipped with ZetaView PMX 120 (Particle Metrix GmbH, Microtrac, Meerbusch, Germany).

    Techniques: Transmission Assay, Microscopy, Expressing, Western Blot